Journal: Antioxidants

Article Title: The Effect of Astaxanthin on Mitochondrial Dynamics in Rat Heart Mitochondria under ISO-Induced Injury

doi: 10.3390/antiox12061247

Figure Lengend Snippet: The influence of AX and ISO change of DRP1, Mfn2, and OPA1 in RHM. ( a ) upper part—Western blot with antibody to DRP1. Tom20 was used for normalization of proteins. Lower part—quantitative characteristic presented as the ratio of DRP1 to Tom20; ( b ) upper part—Western blot with antibody to Mfn2, lower part—quantitative characteristic presented as the ratio of Mfn2 to Tom20; ( c ) upper part—Western blot with antibody to OPA1, lower part—quantitative characteristic presented as the ratio of OPA1 to Tom20. The data are presented as the means ± SDs of four independent experiments. * p < 0.05 significant values compared with control (group 1); # p < 0.05 significant values relative ISO to (group 3).

Article Snippet: The polyclonal anti-Tom20 antibodies (Cell Signaling, Danvers, MA, USA) at dilution 1:1000 were used to normalize the protein.

Techniques: Western Blot

Journal: Antioxidants

Article Title: The Effect of Astaxanthin on Mitochondrial Dynamics in Rat Heart Mitochondria under ISO-Induced Injury

doi: 10.3390/antiox12061247

Figure Lengend Snippet: The influence of AX and ISO change of PHB2 and PINK1 in RHM. ( a ) upper part—Western blot with antibody to PHB2. Tom20 was used for normalization of protein. Lower part—quantitative characteristic presented as the ratio of PHB2 to Tom20; ( b ) upper part—Western blot with antibody to PINK1, lower part—quantitative characteristic presented as the ratio of PINK1 to Tom20. The data are presented as the means ± SDs of four independent experiments. * p < 0.05 significant values compared with control (group 1); # p < 0.05 significant values relative to ISO (group 3).

Article Snippet: The polyclonal anti-Tom20 antibodies (Cell Signaling, Danvers, MA, USA) at dilution 1:1000 were used to normalize the protein.

Techniques: Western Blot